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Construction of a Mesoporous Ceria Hollow Sphere/Enzyme Nanoreactor for Enhanced Cascade Catalytic Antibacterial Therapy

  • Jing Qin
    Jing Qin
    Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, Shaanxi 710049, P. R. China
    More by Jing Qin
  • Youyou Feng
    Youyou Feng
    Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, Shaanxi 710049, P. R. China
    More by Youyou Feng
  • Dong Cheng
    Dong Cheng
    Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, Shaanxi 710049, P. R. China
    More by Dong Cheng
  • Biwu Liu
    Biwu Liu
    Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, Shaanxi 710049, P. R. China
    More by Biwu Liu
  • Zheng Wang
    Zheng Wang
    State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering, College of Chemistry and Chemical Engineering, Ningxia University, Ningxia 750021, P. R. China
    More by Zheng Wang
  • Yongxi Zhao
    Yongxi Zhao
    Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, Shaanxi 710049, P. R. China
    More by Yongxi Zhao
  • , and 
  • Jing Wei*
    Jing Wei
    Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, Shaanxi 710049, P. R. China
    *Email: [email protected]
    More by Jing Wei
Cite this: ACS Appl. Mater. Interfaces 2021, 13, 34, 40302–40314
Publication Date (Web):August 20, 2021
https://doi.org/10.1021/acsami.1c10821
Copyright © 2021 American Chemical Society
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Abstract

Nanozyme has been regarded as one of the antibacterial agents to kill bacteria via a Fenton-like reaction in the presence of H2O2. However, it still suffers drawbacks such as insufficient catalytic activity in near-neutral conditions and the requirement of high H2O2 levels, which would minimize the side effects to healthy tissues. Herein, a mesoporous ceria hollow sphere/enzyme nanoreactor is constructed by loading glucose oxidase in the mesoporous ceria hollow sphere nanozyme. Due to the mesoporous framework, large internal voids, and high specific surface area, the obtained nanoreactor can effectively convert the nontoxic glucose into highly toxic hydroxyl radicals via a cascade catalytic reaction. Moreover, the generated glucose acid can decrease the localized pH value, further boosting the peroxidase-like catalytic performance of mesoporous ceria. The generated hydroxyl radicals could damage severely the cell structure of the bacteria and prevent biofilm formation. Moreover, the in vivo experiments demonstrate that the nanoreactor can efficiently eliminate 99.9% of bacteria in the wound tissues and prevent persistent inflammation without damage to normal tissues in mice. This work provides a rational design of a nanoreactor with enhanced catalytic activity, which can covert glucose to hydroxyl radicals and exhibits potential applications in antibacterial therapy.

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The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acsami.1c10821.

  • Additional results of characterization studies (SEM, TEM, XRD); synthesis of ceria nanoparticles and cubes; stability assay of CeO2/GOx cubes; CAT-like activity assay of mesoporous CeO2 hollow sphere; the relationship between the GOx content and the catalytic property of CeO2/GOx nanoreactors; live and dead cell fluorescence assay (PDF)

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